Supplementation of sugarcane molasses for maximization of ethanol production by Saccharomyces cerevisiae using response surface method

Abstract

Dilute sugarcane molasses containing 19.2% fermentable sugars was supplemented with (NH4)2SO4, KH2PO4, and MgSO4 · 7 H2O to maximize the ethanol production by Saccharomyces cerevisiae TISTR 5596 using a central composite design of response surface method. The maximum ethanol (87.28 g/l) was produced when the dilute molasses was supplemented with 417 mg/l NH+ 4 , 1.88 g/l PO3– 4 and 5 mg/l Mg2+. The Ca2+ contaminated in the molasses was proposed as an indicative index for the requirement of phosphate and Mg2+ supplementation to maximize the ethanol production. © 2019 Science Society of Thailand under Royal Patronage. All rights reserved.