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Polyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression

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dc.contributor.authorChaivisuthangkura P.
dc.contributor.authorTejangkura T.
dc.contributor.authorRukpratanporn S.
dc.contributor.authorLongyant S.
dc.contributor.authorSithigorngul W.
dc.contributor.authorSithigorngul P.
dc.date.accessioned2021-04-05T04:32:22Z
dc.date.available2021-04-05T04:32:22Z
dc.date.issued2006
dc.date.issuedBE2549
dc.description.abstractCapsid protein genes VP1and VP3 of Taura syndrome virus (TSV) were cloned into pGEX-6P-1expression vector and transformed into Escherichia coli BL21. After induction, recombinant VP1 (rVP1) and recombinant VP3 (rVP3) were produced, purified by SDS-PAGE and used for immunization of Swiss mice for antisera production. Anti-rVP1 and anti-rVP3 antisera showed specific immunoreactivities to rVP1 and rVP3 proteins, respectively, by Western blot assay and also yielded good results for detection of TSV in various shrimp tissues by immunohistochemistry. This is the first step towards our target of preparing monoclonal antibodies specific to rVP1 and rVP3 for use in simple immuno-diagnostic test kits for TSV detection and identification. © Inter-Research 2006.
dc.format.mimetypeapplication/pdf
dc.identifier.citationDiseases of Aquatic Organisms. Vol 69, (2006), p.249-253
dc.identifier.doi10.3354/dao069249
dc.identifier.issn1775103
dc.identifier.other2-s2.0-33646505325
dc.identifier.urihttps://swu-dspace2.eval.plus/handle/123456789/5810
dc.rights.holderScopus
dc.subject.otherAntibody
dc.subject.otherAquatic organism
dc.subject.otherClone
dc.subject.otherDisease
dc.subject.otherGene
dc.subject.otherImmunization
dc.subject.otherVirus
dc.subject.otherDecapoda (Crustacea)
dc.subject.otherEscherichia coli
dc.subject.otherLitopenaeus vannamei
dc.subject.otherMiridae
dc.subject.otherTaura syndrome virus
dc.titlePolyclonal antibodies specific for VP1 and VP3 capsid proteins of Taura syndrome virus (TSV) produced via gene cloning and expression
dc.typeArticle
dspace.entity.typePublication
swu.datasource.scopushttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33646505325&doi=10.3354%2fdao069249&partnerID=40&md5=8750167d44efc212530472c493a40217

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