Partial Characterization of a Calcitonin-like Substance from the Brockmann Bodies of the Medaka Oryzias latipes

Abstract

Biochemical natures of calcitonin (CT)-like substance obtained from Brockmann bodies (BB) of the medaka Oryzias latipes (Teleostei) were examined. A crude extract of BB was fractionated by reversed-phase HPLC and screened with anti-salmon CT antisetum by Western blotting. Although 4 fractions showed positive immunoreactivity, CT-like biological activity was detected only in one of these fractions. The molecular weight of the identified CT-like substance was calculated as 20 kDa, which is considerably higher than that (3.5 kDa) of authentic CTs of other vertebrates. © 1995, The Japanese Society of Fisheries Science. All rights reserved.