Electrochemical capillary-flow immunoassay for the detection of anti-sars-cov-2 antibodies

Abstract

Current efforts to tackle the COVID-19 pandemic are limited by the lack of a fast, accurate and low-cost diagnostic test to determine if an individual is infected and contagious. We have developed a capillary-flow immunoassay capable of detecting anti-SARS-CoV-2 nucleocapsid protein antibodies in a 5-µL sample down to 10 ng/mL. The low-cost capillary-flow assay is made of transparency film and adhesive and automatically provides sequential delivery of reagents to a detection zone integrating a screen-printed carbon electrode. Using a portable potentiostat, diagnostic results are obtained within 25 min. © 2020 CBMS-0001