Different professional skill to produce platelet rich plasma may effect the clinical results

Abstract

Background: Previous study of a Manual PRP preparation which stated clearly about the procedure of producing PRP that has platelet concentration higher than baseline. Since there are steps that require skills of performers that prepare the manual PRP, there should be a study of probability that the Manual PRP preparation can minimize human error. Objective: To study the reproducibility of the PRP produced by the manual PRP preparation. The quality of PRP from manual PRP preparation performed by doctor and nurse, including platelet concentration with low levels of white and red blood cells, will be compared. Materials and Methods: In the present study, the performers in this study were the orthopedic resident and the nurse who worked in the orthopaedic out-patient department at HRH Princess Maha Chakri Sirindhorn Medical Center as the representatives of different professions. 12 volunteers were participated. The samples of PRP obtained were then given to the laboratory for analysis to obtain platelet concentration. Results: The PRP performed by doctor had a higher concentration of platelet and lower concentration of WBCs when compared with PRP performed by the nurse. The difference in platelet concentration of PRP from manual PRP preparation between samples performed by doctor and nurse was significant. The difference between the WBC count of PRP from manual PRP preparation carried out by doctor and nurse was significant but the difference of RBC count was not significant. Conclusion: The different medical profession of the performers, which doctors and nurses were the representatives in the present study, gives a statistically significant difference in platelet concentration and the amount of WBCs and RBC. © JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND| 2019.